Contact an Account ManagerAnion exchange chromatography of nucleotides
Summary
Type: HPLC
Application #: 107250
Detection: A) UV, 260nm
B) UV, 260nm
C) UV, 260nm
Conditions: A) eluent: 10mM K phosphate pH 6.3, flow rate 1ml/min
B) buffer A: 2.5mM TRIS/phosphate pH 7.2, buffer B: 2.5mM TRIS/phosphate pH 8.0 + 1.5M KCl, gradient: 5% B to 95% B in 5min, flow rate 1.3ml/min; pressure 120bar
C) buffer A: 20mM TRIS/acetate pH 8.0, buffer B: A) + 1.5M NaCl, gradient: 0% B to 80% B in 30sec, flow rate 2ml/min; pressure 85 bar
Authors: Macherey-Nagel
Source: LC department
Details
Legend: A) Separation of nucleoside monophosphates with isocratic elution, 1.
deoxythymidine monophosphate (dTMP), 2. uridine monophosphate (UMP), 3.
cytidine monophosphate (CMP), 4. inosine monophosphate (